Methods and Materials

Manufacture of AGT CD-CHO

AGT CD-CHO medium was produced by a top spray fluid bed granulation process. All component ingredients were initially processed by hammermilling to establish a uniform particle size. The attritted biochemicals were then transferred to a fluid bed processor that suspended the powder on a column of conditioned air. The levitated powder was sprayed with a fine mist of aqueous solution. Under these controlled conditions, the moistened media particles fuse together with simultaneous water evaporation, producing a porous agglomerate or granule that gives AGT its unique properties. We used fluid bed granulating equipment of three different working capacities: 22L for the 9kg scale, 220L for the 90kg scale and 1100L for the 500kg scale. The finished AGT CD-CHO medium was reconstituted with distilled/deionized water, stirred for 10-15 minutes, and sterile filtered without further supplementation or pH adjustment.