Acknowledgements:

We acknowledge Dr. Hojatollah Vali (McGill University, Montreal, Canada) for the scanning electron photomicrograph of AGT granules. We also acknowledge the following Life Technologies scientists who contributed significantly to this work: Dr. Mark Plavsic for his general support of the AGT project; David Weppner for performing the ANOVA statistical analysis; Rob Keem for conducting and interpreting the analytical data; and Roxanne Mroz for technical expertise in cell culture growth and productivity experiments.

Abbreviations:

AGT™: Advanced Granulation Technology

DPM: Dry powder medium

CHO: Chinese hamster ovary

dhfr: dihydrofolate reductase

ONPG: o-nitrophenyl-B-D-galactopyranoside

HPLC: high pressure liquid chromatography

UV: ultraviolet

ANOVA: analysis of variance