Fundamental and application of aerobic granulation technology for wastewater treatment（15）

Source： | Date: 2011-06-12 | Hits:

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Microbial structure and diversity

Microbial structure

Confocal laser-scanning microscopy (CLSM) has been used with different oligonucleotide probes, specific

fluorochromes, and fluorescent microspheres for studying the microstructure of aerobic granules (Tay et al., 2002d,

2003a; Toh et al., 2003; Jang et al., 2003; Meyer et al., 2003). The obligate aerobic ammonium-oxidizing bacterium

Nitrosomonas spp. was found mainly at a depth of 70 to 100 µm from the granule surface, and aerobic granules

contained channels and pores that penetrated to a depth of 900 µm below the granule surface. The anaerobic

bacterium Bacteroides spp. also detected at a depth of 800 to 900 µm from the granule surface (Tay et al., 2002e),

while a layer of dead microbial cells was located at a depth of 800 to 1000 µm (Toh et al., 2003). Consequently,

smaller granules will be more effective for aerobic wastewater treatment as these granules have more live cells

within a given volume of granules.

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