Homogeneity of Cell Culture Performance 

Cell growth: Cell culture growth supported by individual samples of Lot 83 AGT CD-CHO (500kg) is illustrated in Figure 3. Cell culture performance from the eight samples within Lot 83 showed similar growth patterns consistent with homogenous component distribution.

Productivity: β-glycosidase productivity from cultures grown in samples of AGT CD-CHO (Lot 83) at the 500kg scale also demonstrated homogeneous performance of the cell culture medium (Figure 4). (Similar results were obtained for the 9kg and 90kg lots, data not shown).  These data indicate intrados homogeneity of biological performance AGT-based medium.